The availability of sequences of entire genomes has dramatically increased the number of protein targets, many of which will need to be overexpressed in cells other than the original source of DNA. Gene synthesis often provides a fast and economically efficient approach. The synthetic gene can be optimized for expression and constructed for easy mutational manipulation without regard to the parent genome. Yet design and construction of synthetic genes, especially those coding for large proteins, can be a slow, difficult and confusing process. Through many years of experience in synthetic gene construction, Retrogen has developed a proprietary system by integrating software, instrumentation and protocols that allows us to complete any gene size at a very rapid turnaround time. With this proprietary system Retrogen can offer you complete gene synthesis service, from optimizing the codon to confirming the sequence of the synthetic gene at a very competitive price.
The Advantages of Ordering Custom Gene with Retrogen
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- Design and synthesize all primers
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- Minimize RNA secondary structures
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- Add and/or remove restriction sites
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- Guarantee full sequence verification of both strands
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Specifications for Custom Gene Synthesis
Minimum Sequence Length |
100 base pairs |
Maximum Sequence Length |
15,000 base pairs |
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Gene Optimization Options |
Codon preference for host organism, secondary structure removal, GC content adjustment, addition or removal of restrictions sites |
Starting Material |
A sequence of interest gene in a diskette or email it to us. |
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Gene Construction |
- Using proprietary gene synthesis software to design and construct the entire gene.
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- Clone the synthetic gene in the standard plasmid.
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DNA Sequencing |
Sequence both strands to verify the integrity of the synthetic gene sequence. |
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Final Form of Construct |
20 ug of lyophilized plasmid DNA containing the synthetic gene. |
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Results |
- A complete report of gene construction
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- A plasmid map and printout of assembly report and chromatograms from automated sequencers
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Pricing
Size of Gene |
Price |
50 bp - 400 bp |
$400-600/gene flat rate |
401 bp - 1500 bp |
$1.25-1.60/bp |
1501 bp - 3000 bp |
$1.25-1.80/bp |
>3000 bp |
Inquiry |
Please call for special discount for large gene synthesis projects.
Turnaround time
It will take 2-3 weeks per 1kb. |
Ordering
- Make sure the final gene sequence optimized and modified to your own specifications
- Obtain a price quote from Retrogen custom gene synthesis department at gene@retrogen.com
- Email gene sequences and PO number to gene@retrogen.com or send to the following address:
Retrogen, Inc
Gene Construction Department
6645 Nancy Ridge Dr.
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax)
info@retrogen.com (E-mail)
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