| Starting Material: |
Please send 2-5 µg of plasmid DNA
Email copies of vector map and sequence of the construct with indication of the bases that need to be changed to gene@retrogen.com |
Method: |
We use PCR site-directed method in conjunction with our proprietary protocols
We sequence the mutated region by automated fluorescent sequencing |
Final Form : |
5 µg of lyophilized plasmid DNA with the correct changes |
Documentation:
|
A complete report of strategies and data
A printout of assembly report and chromatograms from automated sequencers |
Price
Each mutant is $300 - $500 which includes both strands sequencing verification. Please call us for a discounted price if you have more than one mutant to be made. |
Turnaround time
Each mutant will take 2-3 weeks, including sequence verification. |
Ordering
Make sure that the correct changes are indicated on the sequence of the construct
Email modified sequence and original sequence to gene@retrogen.com
Enclose 20 µg plasmid DNA and vector map
Send the package priority overnight to the following address:
Retrogen, Inc
Gene Construction Department
6645 Nancy Ridge Drive
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax)
info@retrogen.com (E-mail)
Requirements for Mutagenesis Projects
- Complete DNA sequence of the entire plasmid, both the gene and the vector
- Indication of codon changes needed (specific individual base changes)
- Other changes (deletions, insertions, restriction site changes) clearly indicated
- 2-5 µg of starting template
Note: If the complete sequence of the plasmid is not known, Retrogen will evaluate the project based on the known sequence and determine whether it should be attempted. If the project cannot be completed, the customer will be billed for the full project price to compensate Retrogen for any time, effort and materials used.
Note: All DNA sequence information should only be submitted as a Word file or Plain Text. Genbank or other published references are not acceptable.
Delivery Materials
5 µg of final construct will be sent |
