Recommended Scale of Synthesis and Purification Levels |
|
| Application |
Scale of Synthesis |
Purification |
| |
|
|
| Antisense studies |
1 uM |
RP1 |
| End labelling |
.2 uM |
HPLC, PAGE |
| Gel shift assay |
.2 uM |
HPLC, PAGE |
| Gene synthesis |
.2 uM |
PAGE |
| Hybridization |
.2 uM |
Desalt, RP1 |
| Kinasing |
.2 uM |
HPLC, PAGE |
| Mutagenesis |
.5 uM, .2 uM |
HPLC, PAGE |
| PCR |
.5 nM, .2 uM |
Desalted |
| Qualitative PCR |
.5 uM, .2 uM |
HPLC, PAGE |
| RT-PCR |
.5 uM, .2 uM |
Desalt |
| Sequencing |
.5 uM, .2 uM |
Desalt |
| Modified bases and chemical linkers |
.5 uM, .2 uM |
RP1 |
| Reporter groups (biotin, DIG or fluorescent dyes) |
.5 uM, .2uM |
RP1, HPLC |
|
| |
|
|
| |
|
