Ion's AmpliSeq is a Multiplex PCR-based assay capable of targeting regions of up to 1Mb (1000 Kb). Ion AmpliSeq provides fixed panels designed by leading professionals throughout various fields of study. Using AmpliSeq’s free online design tool, Ion AmpliSeq Designer (www.AmpliSeq.com), you can designate targets for custom panels and sequence just the needed information. You can create your own panels or have your Retrogen Project Manager design a panel to your specifications.
Services / Applications
Ion AmpliSeq Fixed Panels
Inherited Disease Panel
Targeted exons of 300 genes known to be associated with over 700 unique inherited diseases
275x average depth of coverage on an Ion 316 Chip
Comprehensive Cancer Panel
Targeted exons of 409 oncogenes and tumor suppressor genes (including all 50 of the hot spot genes targeted in the “Cancer Hotspot Panel v2”)
350x average depth of coverage on an Ion 318 Chip
Cancer Hotspot Panel v2
Targeted “Hot spot” regions of 50 oncogenes and tumor suppressor genes, with whole exon coverage of KRAS, BRAF and EGFR 98% detection rate for 5% variant frequency
1400x average depth of coverage with up to 16 samples on a single Ion 318 Chip
AmpliSeq Custom Panels
Ion AmpliSeq Community Panels
Ion Torrent has a growing list of community panels which target key regions of genes known to be associated with disease. These panels are designed in collaboration with leading disease researchers from around the world.
BRCA1 and BRCA2 Panel
Targets coding regions of BRCA1 and BRCA2 tumor suppressor genes which are known to be associated with hereditary breast and ovarian cancer. The panel targets 16.25 Kb of sequence in 3 pools.
Colon and Lung Cancer Panel
Targeting hotspot regions in 22 genes implicated in colon and lung cancers.
Dementia Research Gene Panel
Designed as an accurate, quick and cost effective method to identify genetic mutations associated with Early onset Dimentia (EOD). The panel targets mutations in 17 genes causal of dementia.
Custom DNA Panels
Design panels by entering gene names, chromosomal coordinates, or uploading target lists.
Benefits of Retrogen, Inc.'s NGS Services
- Knowledgeable Technical Assistance
- Fast Turnaround for Fixed Panels
- Sequence with the depth of coverage that makes sense for your project
- Multiplexing samples using validated indices lowers your costs
- Library Construction
- Multiple QC steps
- High Quality Data
- Variant Caller for SNP
||High Quality gDNA / FFPE gDNA
|Input Quantity||10 ng (per pool)|
|Input Quality (A260/280)||1.8-2.0 |
|Multiplexing||YES (up to 16 samples)|
|Turnaround Time||2 weeks|