Genomic Services

PCR Amplicons

Next Generation Sequencing provides an extremely efficient deep-sequencing solution for investigating PCR products from almost any source. Using one of several available protocols, Retrogen can sequence amplicons of almost any length. We can sequence your PCR products on a larger scale than traditional Sanger sequencing. Multiplex your samples for even higher throughput. Depending on the application, some types of amplicon sequencing projects are better suited for one platform over another. Please contact your Retrogen NGS Project Manager for assistance in configuring your project.

Services / Applications


PCR Amplicons gDNA, mtDNA and cDNA Derived Amplicons

Clone Checking

Transfection / Infection Checking

Mutagenesis

Vector / Plasmid Insert Sequencing

High Sample Volume Genotyping

Metagenomics (16S rRNA Gene Sequencing)


Benefits of Retrogen, Inc.'s NGS Services

  • Multiplexing samples using validated indices lowers your costs
  • Library Construction
  • Multiple QC steps
  • High Quality Data
  • Cloud-Based Data Analysis (MiSeq runs only)

Platform Specifics

Input requirements, library construction method, data analysis, and data transfer will vary depending on desired platform. Click here to learn more about each platform. Please contact your Retrogen NGS Project Manager with any questions regarding which platform and targeting strategy will best suit your project.

Workflow

  • PCR Amplicon Generation
  • Amplicon QC / Pooling
  • Library Construction
  • Sequencing
  • Data Analysis