Genomic Services
Next-Gen Platforms
Retrogen offers Next Generation Sequencing (NGS) on Illumina's MiSeq and Life Technologies´ Ion Torrent Personal Genome Machine (PGM) platforms. Both platforms support a broad range of applications and library construction protocols. Please visit our resources page for more information.
Platform Comparison Overview
|
MiSeq |
Ion Torrent PGM |
Technology |
Sequencing by Synthesis (SBS) |
Semiconductor Sequencing |
Chemistry | Reversible Terminator Chemistry | Unmodified Nucleotide Incorporation |
Stage | Single lane Flowcell | Ion Chip |
Detection | Fluorescence | Delta pH |
Reads | Paired-End reads up to 2 x 300 | Single end reads up to 400 nt |
Benefits of Retrogen Next Gen Sequencing Services
- Knowledgeable Technical Assistance
- Fast Turnaround
- Sample Preparation through Data Analysis
- Sequence with the depth of coverage that makes sense for your project
- Multiplexing samples using validated indices lowers your costs
- Library Construction
- Multiple QC steps
- High Quality Data
Platform Specifics
|
MiSeq |
PGM |
Flowcells |
1M to 25M Reads |
|
Ion chips | | 400K to 5.5M reads |
Read Lengths | Up to 2 x 300 | Up to 400 |
Data transfer | Flash Drive | Flash Drive |
Data Analysis | Application Dependant | Application Dependant |
Workflows
Workflows will vary depending on your project type and desired platform. Please visit the web page for your application of interest to learn more about their specific workflows. Click Here for a list of all Next-Gen Services offered by Retrogen, Inc.