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Next-Gen Platforms

Retrogen offers Next Generation Sequencing (NGS) on Illumina's MiSeq and Life Technologies´ Ion Torrent Personal Genome Machine (PGM) platforms. Both platforms support a broad range of applications and library construction protocols. Please visit our resources page for more information.

Platform Comparison Overview

	MiSeq	Ion Torrent PGM
Technology	Sequencing by Synthesis (SBS)	Semiconductor Sequencing
Chemistry	Reversible Terminator Chemistry	Unmodified Nucleotide Incorporation
Stage	Single lane Flowcell	Ion Chip
Detection	Fluorescence	Delta pH
Reads	Paired-End reads up to 2 x 300	Single end reads up to 400 nt

Benefits of Retrogen Next Gen Sequencing Services

• Knowledgeable Technical Assistance
• Fast Turnaround
• Sample Preparation through Data Analysis
• Sequence with the depth of coverage that makes sense for your project
• Multiplexing samples using validated indices lowers your costs
• Library Construction
• Multiple QC steps
• High Quality Data

Platform Specifics

	MiSeq	PGM
Flowcells	1M to 25M Reads
Ion chips		400K to 5.5M reads
Read Lengths	Up to 2 x 300	Up to 400
Data transfer	Flash Drive	Flash Drive
Data Analysis	Application Dependant	Application Dependant

Workflows

Workflows will vary depending on your project type and desired platform. Please visit the web page for your application of interest to learn more about their specific workflows. Click Here for a list of all Next-Gen Services offered by Retrogen, Inc.