Genomic Services

Small Genome – Resequencing or De Novo sequencing

infoSmall Genome Sequencing

Available on both Illumina and Ion Torrent Platforms, Retrogen, Inc. offers the small genome sequencing solution you're looking for. With or without reference information, we can deliver high quality sequencing data for your small genome of interest. Contact a Retrogen, Inc. NGS Project Manager today for assistance in configuring your project.

Services / Applications

De novo Assembly Microbial Genomes
Viral Genomes
Non-model Organisms
BAC/YAC Screening
Detection of Recombination Events
Plasmids
Resequencing SNP Discovery
Detection of indel and recombination events
Plasmids

Benefits of Retrogen, Inc.'s Small Genome Sequencing

Knowledgeable Technical Assistance
Fast Turnaround
Small Genomes Can Be Sequenced With Or Without A Reference
Alignment Provided When Reference is Available
De novo Assembly Available
Sequence With the Depth of Coverage that Makes Sense for Your Project
Multiplex Samples to Lower Costs Using Validated Indices
Retrogen, Inc. Will Construct Your Libraries
Multiple QC Steps
High Quality Data
Cloud-Based Data Analysis (Miseq Runs Only)

Platform Specifics

Small Genome Sequencing is available on both the Ion Torrent PGM, and Illumina's MiSeq. Input requirements, library construction method, data analysis and data transfer will vary depending on desired platform. Click here to learn more about each platform, and contact a Retrogen, Inc. NGS Project Manager for any questions regarding which platform will best suit your project.

MiSeq PGM
Input Type gDNA / plasmid DNA gDNA / plasmid DNA
Input Quantity1 1 ng 50-100 ng
Input Quality (A260/280) 1.8-2.0 1.8-2.0
Multiplexing YES (Dual index) YES (Barcode)
Paired-end Reads YES NO
Turnaround Time 2 Weeks 2 Weeks
Data Output .bcf, .bam, .fastq .bam, .bai, .fastq, .sff

1Due to necessary QC processes, over the minimum recommended input quantity is required to be sent prior to library construction.