Genomic Services

Custom Mutagenesis

In vitro site-directed mutagenesis is an important methodology used to study protein structure-function relationships, identify intramolecular regions or amino acids that may mediate these functions, analyze gene expression and modify vectors. Many published mutagenesis methodologies are labor intensive and technically difficult to perform. With many years of experience in mutational manipulation, Retrogen has developed its own methodology that allows us to finish any mutagenesis project at very fast turnaround time and at a very affordable price.

The Benefits of Getting Mutagenesis Projects Done with Retrogen

  • Get Consultation from Our Experienced Scientists
  • Get Your Mutagenesis Projects Done Fast at a Very Competitive Price
  • Sequence the Mutated Region to Ensure a 100% Accuracy

Starting Material:Please send 2-5 µg of plasmid DNA

Email copies of vector map and sequence of the construct with indication of the bases that need to be changed to gene@retrogen.com
Method:We use PCR site-directed method in conjunction with our proprietary protocols

We sequence the mutated region by automated fluorescent sequencing
Final Form :5 µg of lyophilized plasmid DNA with the correct changes
Documentation:A complete report of strategies and data

A printout of assembly report and chromatograms from automated sequencers

Pricing

Each mutant is $300 - $500 which includes both strands sequencing verification. Please call us for a discounted price if you have more than one mutant to be made.

Turnaround time

Each mutant will take 2-3 weeks, including sequence verification.

Note: If the complete sequence of the plasmid is not known, Retrogen will evaluate the project based on the known sequence and determine whether it should be attempted. If the project cannot be completed, the customer will be billed for the full project price to compensate Retrogen for any time, effort and materials used. Note: All DNA sequence information should only be submitted as a Word file or Plain Text. Genbank or other published references are not acceptable.

Send the package priority overnight to the following address:


Retrogen, Inc
Gene Construction Department
6645 Nancy Ridge Dr.
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax)
info@retrogen.com (E-mail)